Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPCHKEPIKKELVTLNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERAMDSNALEKERGITILAKNTAVDYK-----GIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVDAYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQLDFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDYNDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGDLIAV----SGMEDIFVGETVTPVDHQ--DALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARKIEERLMAELQTDVSLRVEPT---NSPDAWTVSGRGELHLSILIENMRRE-GYELQVSRPEVIEKEI-D-------------------GVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPARG-LIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVS----------------IDTGKATTYSIMSIEERGTVFVEPGTEVYEGMIIG-ENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQLTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK 3DEG Chain:C ((2-541)) ------------------KNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDRE-MEAQVLDSMDLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIV---GID----ATDAVRCSAKTGV------------GVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLRKGDKVKVMS-TG--QTYNADRLGIFTP-KQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTLARNPAEKALPGFK---KVKPQVYA-GLFPVSSDD--YE---AFRDALGKLSLNDASLFYEPESSSALGFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPAVNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHG-NQVALTYEIPMAEVVLDFFDRLKSTSRGYASLDYNFKRFQAS---DMV--RVDVLINGERVDALALITHRDNSQNRGRELVEKMKDLIPRQQFDIAIQAAIG-THIIARST------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3DEG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 2687 60024 22.34 123.25 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain C : 0.70 3D Compatibility (PKB) : 22.34 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.409

(partial model without unconserved sides chains): PDB file : Tito_3DEG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3DEG-query.scw PDB file : Tito_Scwrl_3DEG.pdb: