Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence WYAGACDRKSAEEALHRSNKDGSFLIRKSSGHDSKQPYTLVVFFNKRVYNIPVRFIEATKQYALGRKKNGEEYFGSVAEIIRNH 3EAZ Chain:A ((82-156)) WFHGKITREQAERLLY-PPETGLFLVRESTNYP--GDYTLCVSSDGKVEHYRIMYHA--SKLSIDE----EVYFENLMQLVEHY

General information: TITO was launched using: RESULT: Template: 3EAZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 272 -49653 -182.55 -662.04 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -182.55 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.582

(partial model without unconserved sides chains): PDB file : Tito_3EAZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EAZ-query.scw PDB file : Tito_Scwrl_3EAZ.pdb: