Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MREYDIIAIGGGSGGIATMNRAGEHGAQAAVIEEKKLGGTCVNVGCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFDFATLRRNRESYIDRARSSYDGSFKRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVFAWEELPESVAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEMERTNLPLHTHKVPVKLEKT-TDGITIHFEDGT-SHTASQVIWATGRRPNVKGLQLEKAGVTLNERGFIQVDEYQNTVVEGIYALGDVTGEK----ELTPVAIKAGRTLSERLFNGKTTAKMDYSTIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRN---R-QESRFKLITAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTSSEEFVTMR 1ONF Chain:A ((2-495)) --VYDLIVIGGGSGGMAAARRAARHNAKVALVEKSRLGGTCVNVGCVPKKIMFNAASVHDILEN-SRHYGFDT-KFSFNLPLLVERRDKYIQRLNNIYRQNLSKDKVDLYEGTASFL------------EGRNILIAVGNKPVFPPVKGIENTISSDEFFNIK-ESKKIGIVGSGYIAVELINVIKRLGIDSYIFARGNRILRKFDESVINVLENDMKKNNINIVTFADVVEIKKVSDKNLSIHLSDGRIYEHFDHVIYCVGRSPDTENLKLEKLNVETN-NNYIVVDENQRTSVNNIYAVGDCCMVKFYNVQLTPVAINAGRLLADRLFLKK-TRKTNYKLIPTVIFSHPPIGTIGLSEEAAIQIYGKENVKIYESKFTNLFFSVYDIEPELKEKTYLKLVCVGKDELIKGLHIIGLNADEIVQGFAVALKMNATKKDFDETIPIHPTAAEEFLTL-

General information: TITO was launched using: RESULT: Template: 1ONF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2300 -91604 -39.83 -214.03 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : -39.83 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.459

(partial model without unconserved sides chains): PDB file : Tito_1ONF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ONF-query.scw PDB file : Tito_Scwrl_1ONF.pdb: