Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MILKIEDLVMSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKVSLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLLTCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGYNHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGADIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAKSLDFSMKGLTYLDV 3TQV Chain:B ((14-286)) ----------------VPNDIVTRLVRESLAEDIATGDITAQLA--EDIDTTAFCITREEMILCGQDFANEVINQLDKNIQI--TWLYSDAQKVPANARIFELKGNVRSILTAERTILNFIQMLSGTATVTNKLVKLISQYKTKLLDTRKTIPGFRLAQKYAVRCGGGFNHRIGLFDAYLIKENHIRSAGGIAKAVTKAKKLDS-NKVVEVEVTNLDELNQAIAAKADIVMLDNFSGEDIDIAVSIARGKVALEVSGNIDRNSIVAIAKTGVDFISVGAITKHIKAIDLSLQVQ-----

General information: TITO was launched using: RESULT: Template: 3TQV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1409 -194585 -138.10 -712.77 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain B : 0.79 3D Compatibility (PKB) : -138.10 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.497

(partial model without unconserved sides chains): PDB file : Tito_3TQV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TQV-query.scw PDB file : Tito_Scwrl_3TQV.pdb: