Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYKILAGVGSIVRTLFMVFTHGFRKRDTILYPEVPAEEIVPPRYRGRIILTRDPDGEERCVACNLCAVACPVGCISLQKAEKEDGRWYPEFFRINFSRCIFCGMCEEACPTTAIQLTPDFELGEYVRQDLVYEKENLLISGPGKYPDYNFYRVTGMAINGKEKGQAQKESAPIDVRSLLP 5T5I Chain:N ((268-328)) --------------------------------------------------------QDTCQACETCVMVCPCNVLSFPKPEKP--GEKTTKLHKDERFCIYCGACERSCPVTAITVKRN-------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5T5I.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain N - contact count / total energy / energy per contact / energy per residue : 189 -40636 -215.01 -666.16 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain N : 0.57 3D Compatibility (PKB) : -215.01 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.339

(partial model without unconserved sides chains): PDB file : Tito_5T5I.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5T5I-query.scw PDB file : Tito_Scwrl_5T5I.pdb: