Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIIMSFCVACGHKTEQKIPLGDHKVRRVCTHCGNIHYENPKVICGALALWEDKVLLCRRAIEPRYGLWTLPAGYMELFETMEQGAARETREEAEAEIEIEQLYCMYNIPR------I-GQIYVLFKAQLKDGLFGAGEESIECRLFEEHEIPWGELAFPSVEHTLRHYFEDRKQQVFPTHLETLGTRLDHTG 3Q4I Chain:A ((62-199)) ----------------------------------TGYQTPKVDIRAVVFQNEKLLFVKEK---SDGKWALPGGWADVGYTPTEVAAKEVFEETGYEVDHFKLLAIFDKEKHQPSPSATHVYKIFIGCEIIGGEKKTSIETEEVEFFGENELPN-LSIARNTEDQIKEMFAYMKDPQ----------------

General information: TITO was launched using: RESULT: Template: 3Q4I.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 595 1323 2.22 10.10 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 2.22 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.422

(partial model without unconserved sides chains): PDB file : Tito_3Q4I.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3Q4I-query.scw PDB file : Tito_Scwrl_3Q4I.pdb: