Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSIPQSLLEQSIQINIQQALQEDIGDGDITAMLT-PEDEQATATIISREDMVLAGQPWVNALISAYDNTVQVTWLKQEGDRVAANEAFLKLAGSARSLLTVERPALNFIQTLSAVATKTAEYVQHLEGLNTKLLDTRKTLPGLRIAQKYAVTVGGGQNHRLGLFDAFLIKENHIMAAGGIAQAIAKAHQI-APG-KPVEVEVETWDELNQALEAGADIVMLDNFSQQQMIDAVKHVAGRCKLEASGNITIENLREVATTGVDYISMGVLTKDVKAVDLSMRFNA 1X1O Chain:C ((10-284)) -------WQGGLEEALRAWLREDLGQGDLTSLLVVPEDLEGEAVILAKEGGVLAGLWVAERVFALADPRTAFTPLVAEGARVAEGTEVARVRGPLRGILAGERLALNLLQRLSGIATLTRAYVEALAGTKAQILDTRKTTPGLRALEKYAVRVGGGRNHRYGLFDGILLKENHVRAAGGVGEAVRRAK-ARAPHYLKVEVEVRSLEELEEALEAGADLILLDNFPLEALREAVRRVGGRVPLEASGNMTLERAKAAAEAGVDYVSVGALTHSAKALDLSLLVV-

General information: TITO was launched using: RESULT: Template: 1X1O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1419 14733 10.38 54.17 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain C : 0.84 3D Compatibility (PKB) : 10.38 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.421

(partial model without unconserved sides chains): PDB file : Tito_1X1O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1X1O-query.scw PDB file : Tito_Scwrl_1X1O.pdb: