Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE-PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLEDSVLIGPRVNLITVNHLTDPKER--RGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAANATVTKDVPDNTIVAGIPAKIVKPVERII 2P2O Chain:B ((2-184)) -KSEKEKMLAGHLYNPADLE---LVKERERARRLVRLYNETLETEYDKRTGLLKELFG-STGERLFIEPNFRCDYGYNIHVGENFFMNFDGVILDVCEVRIGDHCFIGPGVHIYTATHPLDPHERNSGLEYGKPVVIGHNVWIGGRAVINPGVTIGDNAVIASGAVVTKDVPANAVVGGNPAKVIKWL----

General information: TITO was launched using: RESULT: Template: 2P2O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 935 -14322 -15.32 -79.57 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain B : 0.76 3D Compatibility (PKB) : -15.32 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.659

(partial model without unconserved sides chains): PDB file : Tito_2P2O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2P2O-query.scw PDB file : Tito_Scwrl_2P2O.pdb: