Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSEAYIIDAIRTPRGKGKKDGSLYEVKPITLLTTLLNELQQRHQLDTSKVDDIVLGCVTPIGDQGGDIAKTAAIAAGWNDDVAGVQINRFCASGLEAVNMAAMKVRSGWEDLVVAGGVESMSRIPMGSDG-------G-PWA---------LDPETNLKSSFVPQGVGADLIATLDGYSREDVDNFAVKSQQKAAAAQANGYFDQSVVPVKDHAGVV--ILEKDEFIKGNTTLEGLAKLNPSFEMMGQMGFDAIALQKYPEAQKVNHVHHAGNSSGIVDGAALVLLASEKAVKEQNLKPRAKVLATALVGTDPTIMLTGPAPAARKALEKAGLTIDDIDLFEVNEAFAAVVMRFINELNV--PAEKVNVNGGAIALGHPLGATGAMILGTLLDELERQNKKRGLATLCVGGGMGIATIIERV 1ULQ Chain:G ((2-399)) -PEAWIVEAVRTPIGKHG-G-ALASVRPDDLLAHALSVLVDRSGVPKEEVEDVYAGCANQAGEDNRNVARMALLLAGFPVEVAGCTVNRLCGSGLEAVAQAARAIWAGEGKVYIGSGVESMSRAPYAVPKPERGFPTGNLVMYDTTLGWRFVNPKMQALYGTESMGETAENLAEMYGIRREEQDRFALLSHQKAVRAWEEGRFQDEVVPVPVKRGKEEILVEQDEGPRRDTSLEKLAALRPVFREG---GT-----------------VTAGNSSPLNDGAAAVLLVSDDYAKAHGLRPLARVRAIAVAGVPPRIMGIGPVPATRKALERAGLSFSDLGLIELNEAFAAQALAVLREWSLSMEDQRLNPNGGAIALGHPLGASGARILTTLVHEMRRRKVQFGLATMCIGVGQGIAVVVEG-

General information: TITO was launched using: RESULT: Template: 1ULQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain G - contact count / total energy / energy per contact / energy per residue : 2291 -11942 -5.21 -31.68 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain G : 0.77 3D Compatibility (PKB) : -5.21 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.467

(partial model without unconserved sides chains): PDB file : Tito_1ULQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ULQ-query.scw PDB file : Tito_Scwrl_1ULQ.pdb: