Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIIMSFCVACGHKTEQKIPLGDHKVRRVCTHCGNIHYENPKVICGALALWEDKVLLCRRAIEPRYGLWTLPAGYMELFETMEQGAARETREEAEAEIEIEQLYCMYNIPRI----GQIYVLFKAQLKDGLFGAGEESIECRLFEEHEIPWGELAFPSVEHTLRHYFEDRKQQVFPTHLETLGTRLDHTG 5ANS Chain:A ((21-159)) -------------------------------------GASRLYTLVLVLQPQRVLLGMKKRGFGAGRWNGFGGKVQEGETIEDGARRELQEESGLTVDALHKVGQIVFEFVGEPELMDVHVFCTDSIQGTPV-ESDEMRPCWFQLDQIPF-KDMWPDDSYWFPLLLQKKKFHGYFKFQ-----------

General information: TITO was launched using: RESULT: Template: 5ANS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 615 -21351 -34.72 -158.15 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -34.72 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.319

(partial model without unconserved sides chains): PDB file : Tito_5ANS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ANS-query.scw PDB file : Tito_Scwrl_5ANS.pdb: