Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLYFMYRIIERRLSMKKKLVFPNLFWWGAASSGPQTEG--QYGKVHENVMDYWFKTHPED--FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRL------IKNLETGEPDPKGIAFYNAIIEEAKKNQMDLVMNLHHFDLPVELL--QKYGGW---ESKHVVELFVKFAKTAFTCFGDKVHYWTTFNEPMVIPEAGYL--YAFHYPNLKGKG--KEAVQVIYNLNLASAKVIQLYRSL--ELDGKIGIILNLTPAYPRSNSPEDLEASRFTDDFFNKVFLNPAVKGTFPERLVKQLERDGVLWSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYE-WPERRMNPYRGWEIFPKAIYDIAMIVKEEYGNIPWFISENGMGVENEARFIDENGVIDDVYRIEFYEEHLRWLHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSSGRWYRKVSDNNGFEVEIEE 1E4I Chain:A ((1-447)) -------------TIFQ---FPQDFMWGTATAAYQIEGAYQEDGRGLSIWDTFAH-TP--GKVFNGDNGNVACDSYHRYEEDIRLMKELGIRTYRFSVSWPRIFPNGDG------E-VNQKGLDYYHRVVDLLNDNGIEPFCTLYHWDLPQALQDA---GGWGNR---RTIQAFVQFAETMFREFHGKIQHWLTFNEPWCIAFLSNMLGV--HAPGLT---NLQTAIDVGHHLLVAHGLSVRRFRELGT--SGQIGIAPNVSWAVPYSTSEEDKAACARTISLHSDWFLQPIYQGSYPQFLVDWFAEQGATVPIQDGDMDIIG-EPIDMIGINYYSMSVNRFNP---------EAGFLQSEEIN-MGLPVTDIG-WPVESRGLYEVLHYLQ-KYGNIDIYITENGACINDE---VVN-GKVQDDRRISYMQQHLVQVHRTIHDGLHVKGYMAWSLLDNFEWAEGYNMRFGMIHVDFRTQVRTPKQSYYWYRNVVSNNWLETRR--

General information: TITO was launched using: RESULT: Template: 1E4I.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2486 -24212 -9.74 -56.97 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -9.74 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.462

(partial model without unconserved sides chains): PDB file : Tito_1E4I.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1E4I-query.scw PDB file : Tito_Scwrl_1E4I.pdb: