Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKKTRQTLIGLLVLLLLSTGSYYIKQMPSAPNSPKTNLSQKKQASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTKTVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGHLLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRVRYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ 4QGO Chain:B ((31-224)) --------------------------------------------------------------------------NGIYELNNNQTNLNADVNVKAYVQN---TIDNQQRPSTANAMLDRTIRQYQNRR-------NWKPLGWHQVA-TNDHYGHAVDKGALIAYALAGNFKGWDASVSNPQNVVTQTAHSNQSNQKINRGQNYYESLVRKAVDQNKRVRYRVTPLYRNDTDLVPFAMHLEAKSQDGTLEFNVAIPNTQASYTMDYATGEITL--

General information: TITO was launched using: RESULT: Template: 4QGO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1038 -33030 -31.82 -176.63 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain B : 0.77 3D Compatibility (PKB) : -31.82 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.381

(partial model without unconserved sides chains): PDB file : Tito_4QGO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4QGO-query.scw PDB file : Tito_Scwrl_4QGO.pdb: