Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKLLVALGLAATV-ALVGCNKD-------------KAPETGATTGEHLENSAQQASADIKSAGDQAASDIATATDNASAKIDAATDHAADATAKAAAETEATARKATADTAQAVENAAADVKKDAQH 3AET Chain:B ((175-245)) VTKLLATMGIKVNVCAPLGASPDDLRKLGQAHFNVLMYPETGESAARHLERACKQPFTKIVPIGVGATRDF---------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3AET.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 137 -2620 -19.12 -45.96 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain B : 0.72 3D Compatibility (PKB) : -19.12 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.261

(partial model without unconserved sides chains): PDB file : Tito_3AET.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3AET-query.scw PDB file : Tito_Scwrl_3AET.pdb: