Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLTYGQTKRLRQSKIHASEVITLLLWFHLT-GSRNFKAFYLYWAKPFLCSYFTNLPSYSRFIELKAKYAMYFVALIESLKVHSAGIAFIDSTKLAVCHNKRIHQHRVFADSASRGKTSVDWFYGFK---------IHLICDHIG-RLVSYCITTGNVDDRKVLPDLIEHSKLKGKLFGDRGYVGKNWKSRLAEVGVQLITRVKRNMKPQVLAPFDHAVLKKRGIIEAPFKLMKCQFDLEHGRHRSKMGLLTTIFAALTLYALVLVNGYK-SGIQQILKPIDLNSA- 1DAR Chain:? ((1-328)) -MAVKVEYDLKR-------LRNIGIAAHIDAGKTTTTERILYYTGRAA-VTTCFWKDHRINIIDTPGHVDFTIEVERSMRVLDGAIVVFDSSQGV-----EPQSETVWRQAEKYKVPRIAFANKMDKTGADLWLVIRTMQERLGARPVVMQLPIGREDTFSGIIDVL---RMKAYTYGND--LGTDIREI----------PIPEEYLDQAREYHEKLVEVA---ADFDENIMLKYLEGEEPTEEELVAAIRKGTIDLKITPVFLGSALKNKGVQLLLDAVVDYLPS

General information: TITO was launched using: RESULT: Template: 1DAR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1175 -12989 -11.05 -53.89 target 2D structure prediction score : 0.37 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -11.05 2D Compatibility (Sec. Struct. Predict.) : 0.37 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.206

(partial model without unconserved sides chains): PDB file : Tito_1DAR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1DAR-query.scw PDB file : Tito_Scwrl_1DAR.pdb: