Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVLICDDEPLAVERLSRLVSKM-GHEVVATAHHGQDALEQARLHHPDVILLDIQMPGMNGLVCAEQLSQLNPRPAIVFCTAY--DQHALEAFKSQAQAYLLKPIDPQELEQTFKQLTQLTQAQLSALPKPELLQDLKTQRHQIAAKTYRGVELIPVENIYYFLADQKYVTVRHKNGSVLIDETLKELETEFADQFIRIHRNALVSIAYLDGLELVSSGQYQVRLRGLDERLSVSRRHLSTLRERIHQL 3CZ5 Chain:A ((7-141)) -RIMLVDDHPIVREGYRRLIERRPGYAVVAEAADAGEAYRLYRETTPDIVVMDLTLPGPGGIEATRHIRQWDGAARILIFTMHQGSAFALKAFEAGASGYVTKSSDPAELVQAIEAILAGRRAM-----SPDIAQEIAEER------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3CZ5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 688 -32407 -47.10 -245.50 target 2D structure prediction score : 0.75 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -47.10 2D Compatibility (Sec. Struct. Predict.) : 0.75 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.678

(partial model without unconserved sides chains): PDB file : Tito_3CZ5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3CZ5-query.scw PDB file : Tito_Scwrl_3CZ5.pdb: