Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MCCFVVYGGVLNMSDKKTSLVICFTPLQMLIAEKIVAQQP--SVDLIVIALNNNDKYKYYYNIFQHPNIAK--QYYLFDNNKSRFFKFINIIK-F-NENFKLEKFYENIYISSIDNKYTHNILSKVNFNNLYTYDDGTANIVKNSIYFKQTFE---SKLKDIVLKLIGIRFNLNKIKKLSRKHYTIYKGIQNIVEQT----EYVSIIEKKEEDRNIFIDQEIKIFLGQPLKDIDKNFDTLALKKLLVMEEVNYHFKHPRETDEA-FFEEIKTSYIFEDFFAKELSKY--RKVIIYTLSSTAALNIISLDNVEIRLIKNNTVEIKYP--DLVQLFIKSGATVIDMDSIK 2YK5 Chain:A ((2-321)) -----------------VNLIFCYTILQMKVAERIMAQHPGERFYVVLMSENRNEKYDYYFNQIKDKAEWAYFFHLPYG--LNKSFNFIPTMAELKVKAM-LLPKVKRIYLASLEKVSIAAFLSTYPDAEIKTFDDGTINLIQSSSYLGDEFSVNGTIKRNFARMMIG-DWSIAKTRNASDEHYTIFKGLKNIMDDGRRKMTYLPLFDASELKAGDETGGTVRILLGSPDKEMKE-----ISEKAAKNFNIQYVAPHPRQTYGLSGVTTLNSPYVIEDYILREIKKNPHTRYEIYTFFSGAALTMKDFPNVHVYALKPASLPEDYWLKPVYALFTQSGIPILTFDD--

General information: TITO was launched using: RESULT: Template: 2YK5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1206 -31772 -26.34 -105.20 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -26.34 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.318

(partial model without unconserved sides chains): PDB file : Tito_2YK5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2YK5-query.scw PDB file : Tito_Scwrl_2YK5.pdb: