Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTDLNKKREVNLSFEQDDGAVWVFDGDSHQGTEISHLMMMHSDEYNEDELRVICNHAAFEIDRLRAELEKAKGQAVPDSSHGVILTCEQLRDALEFSAPDLNIESNEFSDEQM-------GTELAIIYQESGHSGEGFYSYYVECPDEGSIKLGESESGAEG 1MIO Chain:C ((372-491)) --------------------------DDYEGREVIPTIKIDADSKNIPEITVTPDEQKYRVVIPEDKVEELKKAGVPLSSYGGMM--KEMHDG-TILIDDMNHHDMEVVLEKLKPDMFFAGIKEKFVIQKGGVLSKQLHSYDYNGPYAG-------------

General information: TITO was launched using: RESULT: Template: 1MIO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 225 7211 32.05 63.81 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain C : 0.67 3D Compatibility (PKB) : 32.05 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.048

(partial model without unconserved sides chains): PDB file : Tito_1MIO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1MIO-query.scw PDB file : Tito_Scwrl_1MIO.pdb: