Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSDVIKDLSELRLSYEQGELYETQVASNPHEQFLGWFNHALAANLHEPYAMSLATASASGRPHVRTVLLRGATEAGYDFYTNYDSQKGIDLAENPYAELLFYWPSLERQVRVGGHVVKIPEQESTDYYHKRPRDSQIAAYISTPQSGKIESRELLQ--QRFQDLQQQVQSHE-VLDKPEFWGGYRLQPDYYEFWQGRPNRLHDRLSYEKIDGQWTLHRLMP 4HMT Chain:A ((33-225)) --------------------YQT-LPADPMSVLHNWLERARRVGIREPRALALATADSQGRPSTRIVVISEISDAGVVFSTHAGSQKGRELLHNPWASGVLYWRETSQQIILNGQAVRLPNAKADDAWLKRP-------YATHPMSSVSRQSEELQDVQAMRNAARQLAELQGPLPRPEGYCVFELRLESLEFWGNGQERLHERLRYDRSDTGWNVRRLQP

General information: TITO was launched using: RESULT: Template: 4HMT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 829 -8154 -9.84 -42.92 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -9.84 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.402

(partial model without unconserved sides chains): PDB file : Tito_4HMT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4HMT-query.scw PDB file : Tito_Scwrl_4HMT.pdb: