Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAELLTLARPYAKAAFAYASEQGATDNWSNALQVLSAAVQDEAFSAYLNRPEHTPAEQVKLFAKVLGEDQSQAVSNFLTLLADNDRLVLLPEIAAEYEQLKSQNNNNVDVVIESAFPLTAEQEQLLKSALEKRFNSTVTVSVEVKPELIAGVVIRAGDQVIDDSALNKLEKMRTRLLA 3PVK Chain:A ((154-253)) ------------------------------------------AYSLYLNSPDAATGQII--FGGV---DNAKYSGSLIALPVTSDRELRISLGSVEVSG-KTINTDNVDVLLDSGTTITYLQQDLADQII-KAFNGKLT---------------------------------------

General information: TITO was launched using: RESULT: Template: 3PVK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 211 -13487 -63.92 -149.85 target 2D structure prediction score : 0.36 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -63.92 2D Compatibility (Sec. Struct. Predict.) : 0.36 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.167

(partial model without unconserved sides chains): PDB file : Tito_3PVK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3PVK-query.scw PDB file : Tito_Scwrl_3PVK.pdb: