Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MISTHILDTNLGKPAAQVEVKLFHTATHELIATAITNQDGRVIDFGI---KNLGKDAYQLEFEVAPYFSSQQI---KTFFPRVCIQFFIEDTNQHYHIPLLISPFAYSTYRGS 2H0F Chain:A ((11-121)) -LTTHILDLTCGKPAANVKIGLKRLG-ESIMKEVYTNNDGRV-DVPLLAGEELMSGEYVMEFHAGDYFASKN-AADQPFLTIVTVRFQLADPDAHYHIPLLLSPFGYQVYRGS

General information: TITO was launched using: RESULT: Template: 2H0F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 435 -4716 -10.84 -45.78 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain A : 0.82 3D Compatibility (PKB) : -10.84 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.426

(partial model without unconserved sides chains): PDB file : Tito_2H0F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2H0F-query.scw PDB file : Tito_Scwrl_2H0F.pdb: