Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTRIYVRASTKDQDAERALADLKNFALSIKGDFNEYIENESGTKLDRPVLNQLLDDSSNGDTLLVESVDRLSRLSQNDFEVLKGRIKDKGLKLVVADLPTTHMLVNSADNITSSILSLVNNMLIDLLATMARLDNDKRRERIKQGLERSGYKPT--GKKADTAKHTRIKELNNKGLTKEEIAKAVGCGVATVYRVIKK 5CY2 Chain:A ((6-180)) -----YARVSTSQQSLDIQIRALKDAGVKANRIFTD---KAS---TDREGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIK-EFDAQGVAVRFID-----------DGISTD--GDMGQMVVTILSAVAQAERRRILERTNEG------KGIKFGRRR-TVDRNVVLTLHQKGTGATEIAHQLSIARSTVYKILE-

General information: TITO was launched using: RESULT: Template: 5CY2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 510 -19166 -37.58 -116.87 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -37.58 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.484

(partial model without unconserved sides chains): PDB file : Tito_5CY2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5CY2-query.scw PDB file : Tito_Scwrl_5CY2.pdb: