Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLIIPAIDLKDGKCVRLKQGRMEDDTVFSDDLVATAQHWVNEGARRLHLVDLNGAFAGTPIHKPVVEAIAKAQPELPIQIGGGIRSLETIEHYLEAGVTFVIIGTKAVQEPEFVEEACKSFAGH-IIVGIDAM-----NGMVATDGWANVTDVKATDLAKRFADAGVSSIVYTDIARDGMMQGVNVEQTVNL-AQYSGLPVIASGGVTNLDDVRNLKGQLGILGAITGRAIYEGTLNLREAQLLLDENRL 5A5W Chain:A ((1-238)) -MIIPALNLIDGTVVRLHQGDYARQRDYGNDPLPRLQDYAAQGAGVLHLVDLTGAKDPAKRQIPLIKTLV-AGVNVPVQVGGGVRTEEDVAALLKAGVARVVIGSTAVKSPDVVKGWFERFGAQALVLALDVRIDEHGTKQVAVSGWQENSGVSLEQLVETYLPVGLKHVLCTDISRAGTLAGSNVSLYEEVCARYPQIAFQSSGGIGDIDDIAALRGT-GVRGVIVGRALLEGKFTVKEA---------

General information: TITO was launched using: RESULT: Template: 5A5W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1299 -75759 -58.32 -327.96 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -58.32 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.604

(partial model without unconserved sides chains): PDB file : Tito_5A5W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5A5W-query.scw PDB file : Tito_Scwrl_5A5W.pdb: