Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKALVAVKRVVDANVKVRVKPDNSGVDLTNVKMSINPFCEIAVEEAVRLKEKG-TVSEIVVVSIGPKEAQEQIRSAMALGADRGILV--ETTDEIGALEVAKILKGVVDAEKPELILLGKQAIDDDSNQVGQMLGALLGAGQGTFASEVKVDGG--KVQVTREIDGG-LQTVELALPAIITTDLRLNEPRYAALPNIMKARKKPLDTKSPADYGVTAGTKLKTIKVEAPAERKAGVQVKSVDELVEKLKNEAKVI 3CLU Chain:C ((1-217)) MKILVAVKQTAALEEDFEIREDGMDVDEDFMMYDLNEWDDFSLEEAMKIKESSDTDVEVVVVSVGPDRVDESLRKCLAKGADRAVRVWDDAAEGSDAIVVGRILTEVIKKEAPDMVFAGVQSSDQAYASTGISVASYLNWPHAAVVADLQYKPGDNKAVIRRELEGGMLQEVEINCPAVLTIQLGINKPRYAS---------KPIEEVSLADIGLSA--------------------------------------

General information: TITO was launched using: RESULT: Template: 3CLU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1093 -17483 -16.00 -86.55 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain C : 0.78 3D Compatibility (PKB) : -16.00 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.520

(partial model without unconserved sides chains): PDB file : Tito_3CLU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3CLU-query.scw PDB file : Tito_Scwrl_3CLU.pdb: