Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQTLIFMHETGKISIDFFKECIMQDIPMSRWLSPLMAFCLSFVIMATLAPTMGIQIDRQIDFWLLWFGTMLLLALPVCYLEIALAKRSKTTALNALSSLTREADSSPKWRVVGCLAVVFIPFLA------------------GNVLSTA------------SNILVAQ---FA--PS-ISSQII-------FVGLAVAALVL----SFIP-RQILILLMTLGVIASIVLANMM-G----S-----TLQPWHWTSV-EFKEWGNATVLALVASGLGLGLYW-QNSVGAVQAQEGATKTVLPIWLAQLIAV--------VAFGFFSL------------------------QAQLPVLTWI---------FTGVMTSALFVQLAREQLAQRQLMPVLQWVIIVVAIAVWAVPEVHN---LFTLIL-MQ--WGL-LICLIYAVFAGWIMKISHLRKSMNFSN-ELFYNLWRIAVRIVLPLSIIVAMIAVIGQSI 2A65 Chain:A ((29-471)) ----------------------------------------------LRFPVQA-AENGGGAFMIPYIIAFLLVGIPLMWIEWAMGRYGGAQGHGTTPAIFYLLWRNRFAKILGVFGLWIPLVVAIYYVYIESWTLGFAIKFLVGLVPEPPPTDPDSILRPFKEFLYSYIGVPKGDEPILKPSLFAYIVFLITMFINVSILIRGISKGIERFAKIAMPTLFILAVFLVIRVFLLETPNGTAADGLNFLWTPDFEKLKDPGVWIAAVGQIFFTLSLGFGAIITYASYV--RKDQDIVLSGLTAATLNEKAEVILGGSISIPAAVAFFGVANAVAIAKAGAFNLGFITLPAIFSQTAGGTFLGFLWFFLLFFAGLTSSIAIMQPMIAFLEDELKLSRKHAVLWTAAIVFFSAHLVMFLNKSLDEMDFWAGTIGVVFFGLTELIIFFWIFGADKAWEEINRGGIIKVPRIYYYVMRYITPAFLAVLLVVWAREY-

General information: TITO was launched using: RESULT: Template: 2A65.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1543 -158750 -102.88 -478.16 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -102.88 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.198

(partial model without unconserved sides chains): PDB file : Tito_2A65.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2A65-query.scw PDB file : Tito_Scwrl_2A65.pdb: