Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MASHYEMFPAVVTRVEQLTPLIKRFTFKRQDGQNFPRFSGGSHIIVKMNE-----QLSNAYSLMSCTQDLSTYQVCVRKDVEGKGG------SVFMHDQCNEGCEIQISEPKNLFPL-AETGNKHILIAGGIGITPFLPQMDELAARGAEYE---LHYAYRSPEHAALLDELTQKHAGH----IFSYVDS------EGSMLNLDELISSQ--------PKGTHVYVCGPKPMIDAVIDCCNKHRYRDEYIHWEQFASTVPEDGEAFTVVLAKSNQEIEVQSNQTILQAIETLNIDVACLCREGVCGTCETAILEGEAEHFDQYLSDAEKASQKSMMICVSRAKGKKLVLDL 1CQX Chain:A ((168-397)) --------------------VITSFILEPADGGPVVNFEPGQYTSVAIDVPALGLQQIRQYSL-SDMPNGRTYRISVKR--EGGGPQPPGYVSNLLHDHVNVGDQVKLAAPYGSFHIDVDAKTPIVLISGGVGLTPMV-SMLKVALQAPPRQVVFVHGARNSAVH-AMRDRLREAAKTYENLDLFVFYDQPLPEDVQGRDYDYPGLVDVKQIEKSILLPDADY-YICGPIPFMRMQHDALKNLGIHEARIHYEVFG-----------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1CQX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 806 7428 9.22 37.71 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 9.22 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.534

(partial model without unconserved sides chains): PDB file : Tito_1CQX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1CQX-query.scw PDB file : Tito_Scwrl_1CQX.pdb: