Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFKHSIFLRIYAGLVILVVLVAVFGYLLVQIINYQRAQEYRESLTDGISYVISEGVARQPGKQQKIDWISDASDLLELPIYYTDASKVELSRTEKKRIEAQKSVVRYDASNSIAYVIIGLRDDPQHYLSIKVDKITERQMKALPIFVLDYLMFYPGQEQEYLAKIQKHFSYPINIQNIQDVNLDSEQIGRLLQDQSVMLYKDSATVRGTTISIVSPIPNHPAQVLVLGPVPMFNWMPLQLSAGITLFSLFLLSLGVYGLILPLERKIRQVRYALNRMKSGDLSLRVPIEGSDEMANLASSYNNMSDHIQRLIEAQRELMRAVSHELRTPVARIRFGTEMLAEE--DDYNHRMHQVDMIDKDIEALNTLIDEIMTYAKLEQGTPSLDFAEIVLFEVLDQVAVETEALKTQKEI---------ELIPPPLYVKVDAERRYLHRVVQNLVGNAVRYC-DNKVRITGGIHNDGMAFVCVEDDGPGIPEQDRKRVFEAFARLDDSRTRASGGYGLGLSIVSRIAYWFGGEIKVDESPSLGGARFIMTWPAHRFKQPPLKTNKKAPA 4ZKI Chain:A ((29-263)) --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------QKIDNDRKQFVSNVSHELRTPLTSLRSYIEALSDGAWKDPEVAPGFLKVTQEETDRMIRMINELLSLSRMDSGTTRVDMELVNINEMFNYVLDRFDMILKKD-NPAKYYTIKREFTKRDLWVEIDTDK--FTQVLDNIMNNAIKYSPDGGVVTCRLLETHNQVIISISDQGLGIPRADLGHVFDRFFRVDK-----QGGTGLGLAISKEVVQMLGGRIWVD-SVEGKGSTFYISLPYE---------------

General information: TITO was launched using: RESULT: Template: 4ZKI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 751 2788 3.71 12.85 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : 3.71 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.521

(partial model without unconserved sides chains): PDB file : Tito_4ZKI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZKI-query.scw PDB file : Tito_Scwrl_4ZKI.pdb: