Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIINSAQLDQLIDLRDRSELHDILDMMIDQFSDDPFAVKLVIFYDKILFHMQAPIKKICVHFETKQ-------WRNKMVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLLLPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVF-DLMDYLGKRHLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVLGSVH-FRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHLEEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRGVFLKFLEKA-ESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVAD-QSPITINLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELKIEFSKQNHYFFVEMYLASRKSKSAY 4QLA Chain:A ((19-442)) -----LDLEEWWGPPELKQKQDT--------SIKPFEIT---FSETMVKELKERIKKRRPFAPPLEGVGFKYGFNSKQLDSWLKYWAEEY-PFAERQKFLNQYPHFKTNIQGLNIHFMRITPKVPK--GVEIVPLLLLHGWPGSVREFYEAIPHLTAVSKDRNFALEIIAPSLPGYGFSDAAVRPGLAAAEVAVIFKNLMARLGYKQYYVQG----------GDWGALIGSAMATFFPKEIIGFHSNMALTLSPAATFLEFVGALFPSLIVEPELANR-------LYPLSEKYSTLLEELGYMHIQATKPDTVGIGLTDSPAGLLAYILEKFST-------------WTNPDLRSKEDGGLSYRWTKDQLIDNLMLYWSTKSIVTSMRLYAESFS---SRHFDLKLDEIQVQVPTWVLQAKH-------ELAYQPPCILKMKYPKLVNASVIEDGGHF-------------LAFELPEIFAKDVLKAIGEFRKLKN--

General information: TITO was launched using: RESULT: Template: 4QLA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2173 -110441 -50.82 -267.41 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -50.82 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.190

(partial model without unconserved sides chains): PDB file : Tito_4QLA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4QLA-query.scw PDB file : Tito_Scwrl_4QLA.pdb: