Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVNPMNLKQAVESI---RTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIGYNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTFAQVKETFEQLSYRTSCKIKVR-DFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLDISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPIHVIEKLFD 3DOO Chain:A ((5-248)) ----------VIGNPISHSLSPLMHHANFQSLNLENTYEAINV-PVNQFQDIKKIISEKSID--GFNVTIPHKERIIPYLDDINEQAKSVGAVNTVLVKDGKWIGYNTDGIGYVNGLKQIYEGIEDAYILILGAGGASKGI--------ANEL--YKIVRPTLTVANRTMSRFNNWSLNINKINLSHAESH-----LDEFDIIINTTP-----------VISLNRLASHTLVSDIVYNPYKTPILIEAEQRGNPIYNGLDMFVHQGAESFKIWT--------------

General information: TITO was launched using: RESULT: Template: 3DOO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1192 -31587 -26.50 -136.74 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -26.50 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.339

(partial model without unconserved sides chains): PDB file : Tito_3DOO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3DOO-query.scw PDB file : Tito_Scwrl_3DOO.pdb: