Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPVVHAFRASQSGLDAGAMKRSSDSHSPPFFFLSSACLAYRTSPMPSPRFARPELNGIITVRRFQRPQLETEAPIRKGDLKEPLEAVAGRVGLRARCSTSAPSADLCLVDDHELVLMGKLVDKGVYYITGGVCIDAEGSDTSSQSPTAALVSSTACGAAPQPPDITVGWCFDARMLLHRSDMN-RSPETPHRLQRAIETLQGCERALDVLPVELLAPFTVHKDSSSGSDLGRAVPPISAANRSQWIPARLATYDEVCSFQDPQVYEHFLKSGAAL----VNLKSD----VYCNDETSSVAVRLSAGAVIDASVAALRGVAASRSGTASSAAGRVHPLVSFCLVRPPGHHCTASQPSGFCLVNNVAIAVQQLRIRHASALPSGPPRIAILDLDVHFGEGTASFVEGACDPASLLYLSLHRYDKRQFYPFDGRGDTAYVGGSRHAASKGSICNVAVHTNGQQPARCEQVISDHLMNSVLEEIFLPRLAEFGPDLVMVSLGFDAAYGDPLGKMAVE-GGFASVLSRLKWWCLHDGRTAGLVVVLEGGYNPEAVAQGV---LSVALALSLPRTDPL----------LQRFLEEKSPKVWSDLRQRQERRHREWQQLREERAEEDVGAAPSGLGSELKPAASDEPEQVQEDALLLDRHKRWCAALVAKVQQIHREAMTRER 5EFJ Chain:D ((8-364)) ---------------------------------------------------------------------------------------------------------------------------------------------------------------P-----ITGLVYDQRMMLHHNMWDSHHPELPQRISRI---------------------FSRHEE-------------LRLLSRCHRIPARLATEEELALCHSSK-HISIIKSSEHMKPRDLNRLGDEYNSIFISNESYTCAL-LAAGSCFNSAQAILTGQV--RNAVA--------------IVRPPGHHAEKDTACGFCFFNTAA-----LTARYAQSITRESLRVLIVDWDVHHGNGTQHIFE---EDDSVLYISLHRYEDGAFFPNSEDANYDKVG-----LGKGRGYNVNIPWNGGKMGDPEYMAAFH-------HLVMPIAREFAPELVLVSAGFDAARGDPLGGFQVTPEGYAHLTHQLM--SLAAGR---VLIILEGGYNLTSISESMSMCTSMLLGDSPPSL-HLTPLKTSATVSINNVLRAHAP-FWSSLR-----------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5EFJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 2159 -21845 -10.12 -65.60 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain D : 0.67 3D Compatibility (PKB) : -10.12 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.441

(partial model without unconserved sides chains): PDB file : Tito_5EFJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EFJ-query.scw PDB file : Tito_Scwrl_5EFJ.pdb: