Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence WTGKSEASNRPPPGRRPEETTWFTSDPSYACGVKIFRPGSEGS----SGLAEITHLNFHEKI-KVLKGQYECEVGPSCSTLRCTMPRGWRLD-IFGKSFPTRRTTYFAENGNPEYNTLASEA-----NIPLY----DCRVVIRFPP-TGGRDTGPLAEATHFN-LGEVVGVLGGRH------------TCQAQGQCANV---------NCNVPKGYTYYSLT--GGPFQTKEKS-HTFQSDA-------- 5IP1 Chain:A ((23-267)) SKVKLTKENIVALLTQGKDLEFEENQNLVAFNFKTFCLENLDQIKKMSIISCLTFLKNRQSIMKVIKQS-DFTFGK--ITIKKTSDRIGATDMTFRRLDSLIRVRLVEETGNSENLNTIKSKIASHPLIQAYGLPLDDAKSVRLAIMLGGS-LPLIASVDSFEMISVVLAIYQDAKYKDLGIDPKKYDTKEALGKVCTVLKSKAFEMNEDQVKKGKEYAAILSSSNPNAKGSVAMEHYSETLNKFYEMFG

General information: TITO was launched using: RESULT: Template: 5IP1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 692 29076 42.02 147.59 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 42.02 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.173

(partial model without unconserved sides chains): PDB file : Tito_5IP1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5IP1-query.scw PDB file : Tito_Scwrl_5IP1.pdb: