Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --MFKTFLKDKEKIVNALQ-LPYSNAKLEATNNLIKLIKHNAFGFRNFEN--FKKERTKFVLSRSSLSSTHYS 2A3D Chain:A ((1-73)) MGSWAEFKQRLAAIKTRLQALGGSEAELAAFEKEIAAFESELQAYKGKGNPEVEALRKEAAAIRDELQAYRHN

General information: TITO was launched using: RESULT: Template: 2A3D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 147 16758 114.00 246.43 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 114.00 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.494

(partial model without unconserved sides chains): PDB file : Tito_2A3D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2A3D-query.scw PDB file : Tito_Scwrl_2A3D.pdb: