Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MEFFGNKPFTQQPQRAITQANQLLDYKSWSEEDRKMFSQLHMREEQVLLAQDYALETARAEDLEQGLERGKVEGRAERKLFTFLDIVRQGLLTSEVASQQLGMSVSEFEALL 5FRF Chain:A ((1-108)) GSHMSAGEPHETDCSEILDHLYEFLDKEMPDSDAVKF--EHHFEESSPCL-EKYGLEQAVKKLVKRAAGQDDVPGDLRAKVMGRLDLIRSGQSVPEHDVAAAPSSSAPQES--

General information: TITO was launched using: RESULT: Template: 5FRF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 251 7977 31.78 74.55 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : 31.78 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.304

(partial model without unconserved sides chains): PDB file : Tito_5FRF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FRF-query.scw PDB file : Tito_Scwrl_5FRF.pdb: