Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTYSHIYQVLFLPKLSIKRWHFLGLVLVNFALSYLSHFELFMVQWKHVIQII 2HEP Chain:A ((1-42)) ----------MISNAKIARINELAAKAKAGVITEEEKAEQQKLRQEYLKGFR

General information: TITO was launched using: RESULT: Template: 2HEP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 62 -5615 -90.56 -133.69 target 2D structure prediction score : 0.79 Monomeric hydrophicity matching model chain A : 0.53 3D Compatibility (PKB) : -90.56 2D Compatibility (Sec. Struct. Predict.) : 0.79 1D Compatibility (Hydrophobicity) : 0.53 QMean score : 0.228

(partial model without unconserved sides chains): PDB file : Tito_2HEP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HEP-query.scw PDB file : Tito_Scwrl_2HEP.pdb: