Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFVWYNLFLKKEREVISMRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPVYDSRTEKLTFGKEVENLEITLHQHTLTITDSFDDQIHISYHPSLSAHHDLITNQNDRTLSLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILRLPKGRTLKGINISAN-RGQTTIIN-ASLENATLNT-NSYILRIEGSRIKNSKLT--TPNIVNIFDTVLTDSQLEST-ENHFHAENIQVHGKVELT-AK-DYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYKTEKTDVKDQLIARSDDNIDLISTPSRR 4OPW Chain:B ((100-225)) -----------------------------------------------------------------------------------------------------------------------------------------------------------IANSPLKGDELKINANSNCLVQLKQKVEVGKLDLNVSGSANMVVNELKTDKLECSINGSGTINLKAGNAEEADYSITTDGEIMAFGVAV-PEVNCKITGKGSAQIHPTDN----LKATI-VGKGNIRYKGPT-----------------------------------------

General information: TITO was launched using: RESULT: Template: 4OPW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 712 26237 36.85 222.34 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain B : 0.57 3D Compatibility (PKB) : 36.85 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.278

(partial model without unconserved sides chains): PDB file : Tito_4OPW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4OPW-query.scw PDB file : Tito_Scwrl_4OPW.pdb: