Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MESRLMKNKRYFFD--------TILIILLLI----------STIFCVSPVFIKLDI---LGTPSHAILTFVLAIPLFY-----ILSQCLHTLLLLVSSIFCKLRPIYF------YFIFVIIIGARKYYRILFHQLMGFSPGIAVFYKESQTTKNLFKFY-----YFLYFTTLISYYFFFTFVYDKPLLLPLISFSIIIALVQKLYRIENQQLFLLKSKVLTILES-----KKNCEFNLQDYHEIWKLQ------SKSELPCVALSYISLIKPYLSESVREQIDLLEVK-------RFKKINHPISLYGMLDVIKLNLYLRHYNEKNKY---------ESMLKKILEVRPD---FVLIEQNIDDS---LNSSQPLSLSLAISEIQLLLEVYMGIKHVSIRR 4FDT Chain:A ((21-425)) MQTKIQKYAGTAMPYPNRTDGMTPFYINHLGRHGARFPTSRKALDKVEKVLVSAQQENGLTSEGMALLSMIRRLSRLFDGQWGKLSKLGETEQEGIAGRMIRNYPQLFSNSAKIEAIATYVPRSINSMDAFLSCMIRHNPALQVQRSEGKQYNHILRFFDLNKSYVNYKEKGDWLPIYKAFVHKKISPVPIMKK--FLLNPEQYLDKEAEEFVMALFSVAAILPDTSIPLNLEDLFTLDEWHRYWQTQNLRQYMSKSSAPVGKMLPVAIAWPLLSEFIRSAQEVISGKSDYQANFRFAHAETVIPFVSLMGIEKTDVQVCRPDSVSVYWKDYEISPMAANVQWLFYRDRDQRIWVKILLNEEAAALPISTACFPYYSWEKTRIFFNQRIEMAKKTLSVFN

General information: TITO was launched using: RESULT: Template: 4FDT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1543 -54694 -35.45 -166.75 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -35.45 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.207

(partial model without unconserved sides chains): PDB file : Tito_4FDT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FDT-query.scw PDB file : Tito_Scwrl_4FDT.pdb: