Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKMKEVKFHL-----ATGLLILTYYLIFNVTSDLDFMVALSDNMYYVFQVLL-------------VLILGTI---------ATIAFVKSEHW----KECGRFQFRWSYLGVFLLSFFLLFVWANLTTYIFPRT-QNGSTVVEVATSLTGISYFVTRILYTSIIAPVSEEVVCRGLLMTSLSKVNRYYLDVLVSAAIFGAMHVLQYGWITTDFIKYFGMGLI--FCMMF-RYTRSIYWAIALHASWNSFLLIVTLLVFGY 5LY8 Chain:A ((10-239)) VKQKSERVYHLDFNQTPTGVTLNNGVTAFPYYEHGNDANVQSGPFGYANGIAYPSTERTASNYWNGPSMSGTIPKNSNGSNTANFQFVNRVNVGTNAAEVGRFEFNLTYQGKIVASLALFDDSASNDQWVFSGTVYDGSQAQMLFFDLLPRNYYRDG-NYNAVITKMGD------QLTFRLDRID------------------LGDGGIETRTVSGFSSVPIDGWTAWFPGFSDQRGWSINWQDSYFEWINVDYW-----

General information: TITO was launched using: RESULT: Template: 5LY8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 845 -45562 -53.92 -233.65 target 2D structure prediction score : 0.18 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -53.92 2D Compatibility (Sec. Struct. Predict.) : 0.18 1D Compatibility (Hydrophobicity) : 0.64 QMean score : -0.011

(partial model without unconserved sides chains): PDB file : Tito_5LY8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5LY8-query.scw PDB file : Tito_Scwrl_5LY8.pdb: