Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKIISHRYFIIV---------FLLVIADQKFSVLV------LRSDLVA-GLSDFAYYLSDMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLF-YSFLALLAFV-A-----WNFVTFFLFPPTRNEISYQHAAPTFTGATAFLMYFFYPVI-AGPIFEDMIYRGLVMT---ALEKGKKWGLDVLGSAV--LFG-VS-HISNH-GWVLTDFVFYMGGGLIFAVLF-RMTKSIYWP-I-----GLHIVYN-GIGQLLMLL--------------------- 3C24 Chain:A ((6-285)) -KNDVGPKTVAILGAGGKMGARITRKIHDSAHHLAAIEIAPEGRDRLQGMGIPLTDG--DGWIDEADVVVLALPDNIIEKVAEDIVPRVRPGTIVLILDAAAPYAGVMPERADITYFIGHPCHPPLFNDETDPAARTDYHGGIA--KQAIVCALMQGPEEHYAIGADICETMWSPVTRTHRVTTEQLAILEPGLSEMVAMPFVETMVHAVDECADRYGIDRQAALDFMIGHLNVEIAMWFGYSPKVAALRLMEFAKDIVVKEDWREALNPAKVKQAAELIAG

General information: TITO was launched using: RESULT: Template: 3C24.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1032 -70178 -68.00 -324.90 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -68.00 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.229

(partial model without unconserved sides chains): PDB file : Tito_3C24.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3C24-query.scw PDB file : Tito_Scwrl_3C24.pdb: