Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIYFDNSATTKPYPEALETYMQVASKILGNPSSLHRLGDQATRILDASRQQIADLIGKKSDEIFFTSGGTEGDNWLIKGV--AFEKAQF--GK-----HIIVSAIEHPAVKESALWL-KSQGFEVDFAPVDK-KGLVDVEALAGLIRHDTILVSIMAVNNEIGSIQPIEAISEFL---------ADKPTISFHVDAVQALAK--IPTEKYLTERVDCATFSSHKFHGVRGVGFVYIKSGKKITPL---LTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQAL---------LN--YPDIFVFSDEENFAPHILTFGIKG--VRGEVIVHAFEDYDIFISTTSACSSKAGKPAGTLIAMGVDKDKAKSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR 3A9X Chain:A ((20-427)) -VYMDYNATTPLEPEVIQAVTEAMKEAWGNPSSSYVAGRKAKDIINTARASLAKMIGGKPQDIIFTSGGTESNNLVIHSTVRCFHEQQTLQG-EGTRPHFITCTVEHDSIRLPLEHLVEDQVAEVTFVPVSKVNGQVEVEDILAAVRPTTCLVTIMLANNETGVIMPISEISRRIKALNQIRAASGLPRVLVHTDAAQALGKRRVDVEDL---GVDFLTIVGHKFYGPR-IGALYVRGVGKLTPLYPMLFGGGQERNFRPGTENTPMIAGLGKAADLVSENCETYEAHMRDIRDYLEERLEAEFGKRIHLNSRFPGVERL-------PNTCNFSIQGSQLRGYMVLAQCQ--TLLASVGA------------------PVDVARNAVRLSVGRSTTRAEVDLIVQDLKQAVNQ-----

General information: TITO was launched using: RESULT: Template: 3A9X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1818 8171 4.49 23.89 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : 4.49 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.428

(partial model without unconserved sides chains): PDB file : Tito_3A9X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3A9X-query.scw PDB file : Tito_Scwrl_3A9X.pdb: