Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKDVSLFLLKKVFKSRLNWIILLLFASVLGVTFYLNSQTANSVSLERELETRLVDRERVINENEEKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLLKEGRWKEAYYLQWQDEEKNYEFVSNDPTASSGLKMGVDRERKIYQALYPLNIKAHTLEFPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPV-SKVTFAMSSLGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKMQDVLFPSLLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVG-----LLFGIQ-TIQ-PLQRIAHLIPFTYLRSVE--ILSGRLS------------------KQIDNVDLNWSMGMVLLPCLIILLLVGILFIERWGSSRKKEVFKV 5DO7 Chain:B ((480-674)) --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------LYYELEDGLYTTGPYFFAKILGELPEHCAYIIIYGMPTYW----LANLR---PG-----L-----------QPFLLHFLLVWLVVFCCRIMALAAAALLPTFHMASFFSNALYNSFYLAGGFMINLSSLWTVPAWISKVSFLRWCFEGLMKIQFSRRTYKMPLGNLTIAVSGDKILSVMELDSYPLYAIYLIVIGLSGGFMVLYYVSLRFIKQKPSQD---

General information: TITO was launched using: RESULT: Template: 5DO7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 500 -67399 -134.80 -403.58 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain B : 0.54 3D Compatibility (PKB) : -134.80 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.162

(partial model without unconserved sides chains): PDB file : Tito_5DO7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5DO7-query.scw PDB file : Tito_Scwrl_5DO7.pdb: