Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLRLVYYQFLHNKKQWLGVSPVIFVSSLVMGLAVNGVINVENNSQVFVGLPDPKPIFMFPIVFGGVTLFFVLSNIINMLVEIFRDDYELLEVLGASRLQLSFLVGGQIFIISSIISFIAYLCSIFVTSNYYYFLQYFFGENILPDIQFQTSAVGCIITVVLISFLAFLSGCFYTFKKIRNRKSSKIRHVLSIVKRILLLAGFSVIWLLSLQQIFQDSTILAKAQIIFNIVILDIVIIYQLSPFIQSCFIKLLSIIIFRN-NFMFIVSKWNLLYC-KSYIKSISAAITGAILLISSFQMISQNILSQFQDDSDLELKVAFIVYVGAPILIVLANIISIAFLSSHQERIEIQQFEILGTSNYQMVKIKVGEAIFLTFVTSLIAFLLNIKIMALIYYSLEDILIDDMNLLGLILPNFIVSIILFILIFITKSSYFIFKNAKIIS--- 5TZJ Chain:A ((1-349)) -MKFSVIVPTYNSEK--------YITELLNSLA---------------KQDFPKTEFEVVVVDDCST------DQTLQIVEKYRNKLN----LKVSQLETN--SGGPGKPRNVALKQAEGEFVLFVDSDDYI------NKETLKDAAAFIDEHHS--DVLLIK-----------MKGVNGRGVPQ--SMFKETAPEVTLLNSRIIYTLSPTKIYR-TALLKDNDIYF-------------PEELKSAEDQLFTMKAYLNANRISVLSDKAYYYATKREGEHMSSAYVSPEDFYEVMRLIAVEILN-----ADLE--------------EAHKDQILAEFLNRHFSFSRTNGFSLKVKLEEQPQWIN-ALGDFIQAVPERVDALVMSKLRPLLHYARAK----DIDNYRTVEESYRQGQYYRFDIVDGKLNIQFNEGEPYFEGID

General information: TITO was launched using: RESULT: Template: 5TZJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1861 -208259 -111.91 -605.40 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -111.91 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.311

(partial model without unconserved sides chains): PDB file : Tito_5TZJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5TZJ-query.scw PDB file : Tito_Scwrl_5TZJ.pdb: