Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---MKRIIPVYIFQQVNVLLVSLYLLKFLCIGEL-----TILQILYGSSLISFLWMYGQRKQAHKVNMKSRMKWLGVEFVSLLIISLCFSLIHAQG-STNQANLIGLQHQIP-WFSFLLFLI-----------------NASMVEEFLYREILCNLVRKLDIRVALTSV----------------LFALAHHTGTILAWCLYVS----LGMFLGMVRYKSDLWGS---------------------------MGLHLVWNLLVYSLLLF------------ 5E04 Chain:A ((116-399)) SADWKAIGAYILGFAIPIILKALYMLSTRGRQTVKDNKGTRIRFKDDSSFEEVNGIRKPKHLYVSMPTAQKAEEITPGRFRTIACGLFPAQVKARNIISPVMGVIGFGFFVKDWMDRIEEFLAAECPFLPKPKVASEAFMSTNKMYFLNRQRQVNESKVQDIIDLIDHAETESATLFTEIATPHSVWVFACAPDRCPPTALYVAGVPELGAFFSILQDMRNTIMASKSVGTAEEKLKKKSAFYQSYLRRTQSMGIQLDQKIIILYMLSWGKEAVNHFHLGD

General information: TITO was launched using: RESULT: Template: 5E04.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 752 -94273 -125.36 -483.45 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -125.36 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.218

(partial model without unconserved sides chains): PDB file : Tito_5E04.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5E04-query.scw PDB file : Tito_Scwrl_5E04.pdb: