Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVEQIPVGHNSGSFFLFLLLRLLLSPLLRNSISFLTSQGIPWKLSNNKTKPIDKPTASKSIATNPLLLHLR--------------------------- 1VFI Chain:A ((1-95)) ISEFAPVDCKGQCTTPCEPLTACKEKCAES---CETSADKKTCRRNCKKADCEPQDKVCDACRMKCHKACRAANCASECPKHEHKSDTCRACMKTNCK

General information: TITO was launched using: RESULT: Template: 1VFI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 163 -7867 -48.26 -115.68 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -48.26 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.301

(partial model without unconserved sides chains): PDB file : Tito_1VFI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VFI-query.scw PDB file : Tito_Scwrl_1VFI.pdb: