Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------MRFEVLSTSLIVTKIVFFVR 2ME3 Chain:A ((657-683)) EKDLLALDSWKNLWNWFNITNWLWYIK

General information: TITO was launched using: RESULT: Template: 2ME3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 13 35 2.69 1.75 target 2D structure prediction score : 0.20 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 2.69 2D Compatibility (Sec. Struct. Predict.) : 0.20 1D Compatibility (Hydrophobicity) : 0.69 QMean score : -0.222

(partial model without unconserved sides chains): PDB file : Tito_2ME3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ME3-query.scw PDB file : Tito_Scwrl_2ME3.pdb: