Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTANIMTTLKNQITDALKTSMRAKDMATVTVLRSL-QAAIKQIEVDERIELDDAQVLAVIEK-QIKQRKESIKAFEGAGRDDLASKEQAEAEVLSQFLPEA--------MTEEELDSLIEQTIAAQEATSMKDMGKVMNSLRPIIAGRADPAQVSAKIKAKLA 3RG2 Chain:A ((486-605)) -------------------------LRAVQVRRFLREQGIAEFKLPDRVECVDSLPLTAVGKVDKKQLRQWLASRASAGRASIPASKAALREVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTI------------------

General information: TITO was launched using: RESULT: Template: 3RG2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 185 7562 40.87 68.74 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 40.87 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.508

(partial model without unconserved sides chains): PDB file : Tito_3RG2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3RG2-query.scw PDB file : Tito_Scwrl_3RG2.pdb: