Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------------------MTYFMTKSA--VKRKNSDIKKPLHAQTGVVYRHSVEVNQMN---TNNLSNQQQIIQSWFEPALHTLKALIKKCEENLERIKGDTKNAAVRREEFKEALARQHCITYNHAEEIIRSLSRVDRIRFLGSTYIQIKEGGEA-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- 4CI7 Chain:A ((4-470)) GSHKTLDGVETAEYSESYLQYLEDVKNGDTAKYNGVIPFPHEMEGTTLRKSSVAYNPMDLGLTTPAKNQGSLNTAWSFSGMSTLEAYLKLKGYG----------------------------TYDLSEEHLRWWA----------------TGGKYGWNLDDMSGSSNVTAIGYLTAWAGPKLEKDIPYNLKSEAQGATKPSNMDTAPTQFNVTDVVRLNKDKETVKNAIMQYGSVTSGYAHYSTYFNKDETAYNCTNKRAPLNHAVAIVGWDDNYSKDNFASDVKPESNGAWLVKSSWGEFNSMKGFFWISYEDKTLLTDTDNYAMKSVSKPDSDKKMYQLEYAGLSKIMSNKVTAANVFDFSRDSEKLDSVMFETDSVGAKYEVYYAPVVNGVPQNNSMTKLASGTVSYSGYINVPTNSYSLPKGKGAIVVVIDNTANPNREKSTLAYETDIDGYYLYEAKANLGESYILQNNKFEDINTYSEFSPCNFVIKAITKTS

General information: TITO was launched using: RESULT: Template: 4CI7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 113 13409 118.66 150.66 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.55 3D Compatibility (PKB) : 118.66 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.242

(partial model without unconserved sides chains): PDB file : Tito_4CI7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4CI7-query.scw PDB file : Tito_Scwrl_4CI7.pdb: