Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMSSVSIAEYRKLFPIKKNKKRRSAKQVARQLSVGEMVLATHLRACKI---GFEQEYKFHPKRKWRADFLITGTKILVEVEVGIWSGGRHTRGKGYLGDMEKYNSAAMMGFTVLRFSTEQVKSGV--AIKQIEQLVG 3HRL Chain:A ((3-98)) ------------------------------AMSEAEAKLWQHLRAGRLNGYKFRRQQP---MGNYIVDFMCVTPKLIVEADG--------------VYDHARTVYLNSLGFTVLRFWNHEILQQTNDVLAEILRVL-

General information: TITO was launched using: RESULT: Template: 3HRL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 326 -7370 -22.61 -87.73 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -22.61 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.510

(partial model without unconserved sides chains): PDB file : Tito_3HRL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3HRL-query.scw PDB file : Tito_Scwrl_3HRL.pdb: