Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSTPEDISQNKPLLWLMAAACGLCAGVNYYCQPLIHSIQQDF-AVTQAQAALTVTFAQVSYALGLLFIVPMGDIVNKAKAIP---FLMMLCAVGLFTSAFAVNL-PMLWIGTILAGLFS-VAAQVLIPLATMTVKP--EKTGEIIGFLMSGLLVGILLSTSLAGLFSNLFHWKVVYAISGILMLLLAYTLKSRLPYVMRM-KLN-Y--GQ---IFVSMAQLLKEEKRLLLRALTGAFAFASVSILYSTIALLMTSAH---HLPDLFIGLV-PLVGIFGALSTRYIGKYADQGYTRLLTWMGCGLFLLSWICFYFGQTLLSAYVIGFALIQLALALVHTSNQSIIFR-LRPDAKSRINAIYMTTY-FIGGAAGSALGIFAWNRGEWTMTCLAGVGLVVFCILFSMIDAFLQKKQMA 2CFQ Chain:A ((10-393)) --------------WMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSDTGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYNILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALGASIVGIMFT-INNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFRQPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIMFFAPLIINRIGGKNALLLAGTIMSVRIIGSSFAT-SALEVVILKTLHMFEVPFLLVGCFKYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGFT---------------

General information: TITO was launched using: RESULT: Template: 2CFQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1695 -260538 -153.71 -717.74 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -153.71 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.263

(partial model without unconserved sides chains): PDB file : Tito_2CFQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2CFQ-query.scw PDB file : Tito_Scwrl_2CFQ.pdb: