Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKKGSFMPASTSQYYTRTAQILHWVMAIIFITAWVIGFYSGNFL-SYD-----------VDGSFKGDVITLHKNIATTIIFLVVIRLFWRYTHPAPQLPDTMSPTMKTLAHIGHLLLYVILVALPVTGCLFSWSAGHPA------PVLYLFEIPRLVQ-----------DNPELLAIVKPLHIYISWFAGFLIVGHVLAALKHHFVDKDNVLNSMTKQPK 3CX5 Chain:N ((35-204)) -------------------------LLGLCLVIQIVTGIFMAMHYSSNIELAFSSVEHIMRDVHNGYILRYLHANGASFFFMVMFMHMAKGLYYGSYRSPR-------VTLWNVGVIIFILTIATAFLGYCCVYGQMSHWGATVITNLFS--AIPFVGNDIVSWLWGGFSVSNPTIQRFFALHYLVPFIIAAMVIMHLMALHIH-----------------

General information: TITO was launched using: RESULT: Template: 3CX5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain N - contact count / total energy / energy per contact / energy per residue : 371 -58462 -157.58 -414.62 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain N : 0.61 3D Compatibility (PKB) : -157.58 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.169

(partial model without unconserved sides chains): PDB file : Tito_3CX5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3CX5-query.scw PDB file : Tito_Scwrl_3CX5.pdb: