Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKILVRNLDRSVTEAEVLELFKAYGKVESCVVVTDKDTGKSKGFGFVEMPNPHEAIKAIKGLNTLKVKGYGIRVKAAEE 2DNZ Chain:A ((231-307)) --LYVGSLHFNITEDMLRGIFEPFGKIDNIVLMKDSDTGRSKGYGFITFSDSECARRALEQLNGFELAGRPMRVGHVTE

General information: TITO was launched using: RESULT: Template: 2DNZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 290 -62447 -215.33 -810.99 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.82 3D Compatibility (PKB) : -215.33 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.700

(partial model without unconserved sides chains): PDB file : Tito_2DNZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2DNZ-query.scw PDB file : Tito_Scwrl_2DNZ.pdb: