Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTAIQPLFTELEPMPKALADAPIGIFDSGIGGMSVAAEIAKYLPNERIVYYADTAYVPYGPRSDEEIRELTARAVDWLYRQGCKIAVVACNTASAFSLDHLREHYGEHFPIVGLV-PALKPAVLQTRSKVVAVLATPATFRGQLIKDVVEKFAVPAGVKVMTLTSLELVPCVEAGQQMSPVCLN-ALREVLQPAVEQGADYLVLGCTHYPFLNEAIHHLFDNQFTLVDSGLAVARQTARILIKNELLCDQIRQNVARIECYVSGNNADALQPVLQNMIPQELTWTLHNLS 2JFQ Chain:A ((22-239)) -------------------NKPIGVIDSGVGGLTVAKEIMRQLPNETIYYLGDIGRCPYGPRPGEQVKQYTVEIARKLMEFDIKMLVIACNTATAVALEYLQKTLS--ISVIGVIEPGARTAIMTTRNQNVLVLGTEGTIKSEAYRTHIKR--INPHVEVHGVACPGFVPLVEQMRYSDPTITSIVIHQTLKRWRNSESDTVILGCTHYPLLYKPIYDYFGGKKTVISSGLETAREVSALL-------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2JFQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1293 -105466 -81.57 -488.27 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -81.57 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.650

(partial model without unconserved sides chains): PDB file : Tito_2JFQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2JFQ-query.scw PDB file : Tito_Scwrl_2JFQ.pdb: